植物乳杆菌诱导绵羊瘤胃上皮细胞SBD-1表达的信号通路途径初探

doi:10.11843/j.issn.0366-6964.2016.05.021

Abstract

Abstract:

This study aims to explore the possible approach that lactobacilli induce the expression of SBD-1 in sheep rumen epithelium cells. Real-time fluorescence quantitative PCR（RT-qPCR）was conducted in this study to determine mRNA expressive variation of the toll like receptor 2 (TLR2) and its related factors in the established model to induce SBD-1. Three signaling pathway inhibitors were chosen which named NF-κB signaling pathway inhibitor PDTC, ERK 1/2 signaling pathway inhibitor PD98059 and JNK signaling pathway inhibitor SP600125, respectively. The tested cells were divided into 8 groups : cell group: without treatment; positive groups:L.plantarum P-8; PDTC groups : PDTC pretreatment cells only; PDTC+ L.plantarum P-8 group:PDTC+L.plantarum P-8;PD98059 group:PD98059;SP600125 group:SP600125;PD98059+ L.plantarum P-8 group:PD98059+L.plantarum P-8;SP600125+L.plantarum P-8 group: SP600125+ L.plantarum P-8.RT-qPCR method was used for detecting the expression levels of SBD-1 mRNA. The results indicated that the mRNA expression of TLR2, MyD88, NF-κB, ERK1/2 and JNK had significant increase compared with the cell groups (P<0.01) after the sheep rumen epithelial cells were induced by L.plantarum P-8, PD98059 and SP600125 could significantly inhibited the mRNA expression of SBD-1 in cells (P<0.01) with pretreatment that adding the inhibitors and were induced by L.plantarum P-8, however, the expression of SBD-1 was only inhibited significantly by the inhibitor PDTC (P<0.05). The results in this paper implied that L.plantarum P-8 could promote the mRNA expression of TLR2, MyD88, NF-κB, JNK and ERK1/2. To add NF-κB signaling pathway inhibitor PDTC, ERK 1/2 signaling pathway inhibitor PD98059 and JNK signaling pathway inhibitor SP600125 could inhibit the effect of L.plantarum P-8 on inducing SBD-1 mRNA. Thus, L.plantarum P-8 could improve the SBD-1 expression by activating the signaling pathways of NF-κB, JNK and ERK1/2.

CLC Number:

S826
S852

FAN Yan-ru，JIN Xin，TIAN Qiao-zhen，ZHANG Man，LIU Jiao，YANG Yin-feng. Preliminary Study on the Pathway of SBD-1 Expression in Sheep Rumen Epithelial Cells Induced by Lactobacillus plantarum[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 1026-1032.

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Preliminary Study on the Pathway of SBD-1 Expression in Sheep Rumen Epithelial Cells Induced by Lactobacillus plantarum

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